Mastering Antibody Purification Techniques: A Comprehensive Guide
Protein A/G Chromatography: Protein A and Protein G are affinity chromatography resins widely used for antibody purification. They selectively bind to the Fc region of immunoglobulins, allowing for efficient purification from crude samples. This technique is suitable for purifying monoclonal antibodies from cell culture supernatants or ascites fluid.
